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Sigma-Aldrich

iTRAQ® Reagent - Multiplex Buffer Kit

Synonym(s):

isobaric tags for relative and absolute quantitation, buffer kit 

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

shipped in

dry ice

storage temp.

−20°C

General description

Contains SDS – Denaturant, TCEP Reducing Reagent, MMTS Cysteine Blocking Reagent, TEAB Buffer and Isopropanol, sufficient material for either two 8-plex Multiplex Kits, three 4-plex Kits, or four mTRAQ® 10 Assay Kits.
The iTRAQ® Reagents are a set of isobaric, multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples at the same time, allowing for simultaneous identification and quantitation, both relative and absolute, while preserving important post-translational modification (PTM) information. The iTRAQ® reagents can be used to study the differential expression of proteins in perturbed systems.

Application

iTRAQ® Reagent - Multiplex Buffer Kit has been used in the isobaric tag for relative and absolute quantitation (iTRAQ) labeling of protein samples.

Analysis Note

To view the Chemistry Quick Reference Card for the iTRAQ® Reagents, please visit this Chemistry Quick Reference Card link.

Legal Information

MTraq is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12.0 °C - closed cup


Certificates of Analysis (COA)

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Lynn R Zieske
Journal of experimental botany, 57(7), 1501-1508 (2006-04-01)
Proteomic research includes the characterization of protein mixtures in order to understand complex biological systems and determine relationships between proteins, their function, and protein-protein interactions. Often the goal of such research is to monitor changes of proteins in perturbed systems
Hongmei Lu et al.
Clinical proteomics, 16, 33-33 (2019-08-07)
Diabetic nephropathy is the most frequent cause of end-stage renal disease worldwide. Identification of biomarkers for diabetic nephropathy for early diagnosis may be the key to avoiding damage from this condition. Proteomic iTRAQ technology was first used to identify differentially

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