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Sigma-Aldrich

iTRAQ® Reagents Multiplex Kit

Synonym(s):

isobaric tags for relative and absolute quantitation 

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.24

shipped in

dry ice

storage temp.

−20°C

General description

Contains iTRAQ® reagents 114, 115, 116, 117, the appropriate buffers and reagents for ten 2-plex, six 3-plex, or five 4-plex assays. 10-PK trypsin is sold separately. Each individual reagent is capable of labeling up to 100 μg of protein.

Application

The iTRAQ® Reagents Multiplex Kit provides iTRAQ® reagents and protocol to label peptides after digestion. This widely used generic labeling kit can be used for virtually any workflow.
iTRAQ® Reagents Multiplex Kit has been used for the labeling of peptides for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.

Analysis Note

To view the general Protocol for iTRAQ Reagents, please visit this Protocol link.

To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

The Certificate of Analysis (CofA) for your specific lot is accessible by entering the product number and lot number at this CofA access link.

Legal Information

iTraq is a registered trademark of AB Sciex Pte. Ltd.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

35.6 °F - closed cup

Flash Point(C)

2.0 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sonia Osorio et al.
Plant physiology, 157(1), 405-425 (2011-07-29)
Tomato (Solanum lycopersicum) is an established model to study fleshy fruit development and ripening. Tomato ripening is regulated independently and cooperatively by ethylene and transcription factors, including nonripening (NOR) and ripening-inhibitor (RIN). Mutations of NOR, RIN, and the ethylene receptor
Quantitative proteomic approach to study subcellular localization of membrane proteins.
Sadowski PG, et al.
Nature Protocols, 1, 1778-1789 (2006)
Izhak Michaelevski et al.
Molecular & cellular proteomics : MCP, 9(5), 976-987 (2009-12-04)
Investigations of the molecular mechanisms underlying responses to nerve injury have highlighted the importance of axonal transport systems. To obtain a comprehensive view of the protein ensembles associated with axonal transport in injured axons, we analyzed the protein compositions of
Emilie Ernoult et al.
Proteome science, 6, 27-27 (2008-10-15)
The development of mass spectrometric techniques and fractionation methods now allows the investigation of very complex protein mixtures ranging from subcellular structures to tissues. Nevertheless, this work is particularly difficult due to the wide dynamic range of protein concentration in
Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.
Zeiner CA, et al.
PLoS ONE, 11, e0157844-e0157844 (2016)

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