SRP0222

PTPmu Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): PTPRL1, PTPRM, Protein tyrosine phosphatase mu, R-PTP-MU, hR-PTPu
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 56.2 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: BC051651
• UniProt accession no.: P28827
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PTPRM(5797)

Description

General description

Protein tyrosine phosphatase. Catalytic domain, (amino acids 839-1100), (Gene bank accession number BC051651) with N-terminal GST tag, MW = 56.2 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable