Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

PKH67GL

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Synonym(s):

Green PKH membrane labeling kit

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

Quality Level

200

packaging

pkg of 1 kit

storage condition

protect from light

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Application

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used:
  • To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition.
  • To label apoptotic cells, in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells.
  • In fluorescence imaging.

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Description
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)
Increased expression of inducible HSP70 in apoptotic cells is correlated with their efficacy for antitumor vaccine therapy
Masse D, et al.
International Journal of Cancer. Journal International Du Cancer, 111(4), 575-583 (2004)
Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Z M Wu et al.
Placenta, 33(3), 188-194 (2012-01-04)
As human blastocyst-derived extravillous trophoblasts (EVTs) invade the early decidua, they are positioned to interact with immune cells and resident decidual cells, and remodel spiral arteries into high capacity vessels that increase blood flow to the developing fetal-placental unit. Shallow
View All Related Papers

Articles

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

A video about how you can use fluorescent cell tracking dyes in combination with flow and image cytometry to study interactions and fates of different cell types in vitro and in vivo.

Cell Tracking with Lipophilic Membrane Dyes

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service