MIRRM02
MystiCq® microRNA® SYBR® Green qPCR ReadyMix™
with ROX™, formulation for miRNA RT-qPCR
Synonym(s):
SYBR® Green-based miRNA qPCR master mix, miRNA qPCR, microRNA qPCR master mix with ROX
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
technique(s)
qPCR: suitable
compatibility
ABI 5700
ABI 7000
ABI 7300
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOne
ABI StepOnePlus
storage temp.
−20°C
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General description
The MystiCq® microRNA SYBR® Green qPCR ReadyMix™ is one of the integral components of the MystiCq microRNA qPCR Assay System. This master mix is a specially formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.
Application
MystiCq® microRNA SYBR® Green qPCR ReadyMix™ has been used in a quantitative real-time polymerase chain reaction (qPCR) to quantify miRNA expression levels in different cell lines.
Features and Benefits
- Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer
- 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer
- Passive reference dye (if required) included at the optimal concentration
- This master mix can readily amplify fragments between 400 and 500 bp; however, for best results, amplicon size should be limited to 80 - 200 bp
Packaging
Default reaction volume is 50 μL
100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL
100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL
Other Notes
MystiCq® microRNA SYBR® Green qPCR ReadyMix™, ROX™, is compatible with the following platforms: Applied Biosystems 5700, Applied Biosystems 7000, Applied Biosystems 7300, Applied Biosystems 7700, Applied Biosystems 7900, Applied Biosystems 7900 HT Fast, Applied Biosystems 7900 HT, Applied Biosystems StepOnePlus, Applied Biosystems StepOne.
Legal Information
MystiCq is a registered trademark of QIAGEN Beverly Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hanying Ding et al.
Nature communications, 7, 11533-11533 (2016-06-01)
Visceral adiposity is strongly associated with metabolic disease risk, whereas subcutaneous adiposity is comparatively benign. However, their relative physiological importance in energy homeostasis remains unclear. Here, we show that after 24-h fasting, the subcutaneous adipose tissue of mice acquires key
Sadegh Saghafinia et al.
Cancer discovery, 11(10), 2638-2657 (2021-04-30)
Pancreatic neuroendocrine tumors (PanNET) comprise two molecular subtypes, relatively benign islet tumors (IT) and invasive, metastasis-like primary (MLP) tumors. Until now, the origin of aggressive MLP tumors has been obscure. Herein, using multi-omics approaches, we revealed that MLP tumors arise
Hanying Ding et al.
Nature communications, 7, 11533-11533 (2016-06-01)
Visceral adiposity is strongly associated with metabolic disease risk, whereas subcutaneous adiposity is comparatively benign. However, their relative physiological importance in energy homeostasis remains unclear. Here, we show that after 24-h fasting, the subcutaneous adipose tissue of mice acquires key
Sadegh Saghafinia et al.
Cancer discovery, 11(10), 2638-2657 (2021-04-30)
Pancreatic neuroendocrine tumors (PanNET) comprise two molecular subtypes, relatively benign islet tumors (IT) and invasive, metastasis-like primary (MLP) tumors. Until now, the origin of aggressive MLP tumors has been obscure. Herein, using multi-omics approaches, we revealed that MLP tumors arise
Katarzyna Goljanek-Whysall et al.
Development (Cambridge, England), 141(17), 3378-3387 (2014-08-01)
Myogenesis involves the stable commitment of progenitor cells followed by the execution of myogenic differentiation, processes that are coordinated by myogenic regulatory factors, microRNAs and BAF chromatin remodeling complexes. BAF60a, BAF60b and BAF60c are structural subunits of the BAF complex
Protocols
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
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