A6270

Azure A chloride

Dye content ≥70 %

• Synonym(s): Azure eosin methylene blue; 7-Amino-N,N-dimethyl-3H-phenothiazin-3-iminium chloride
• Empirical Formula (Hill Notation): C₁₄H₁₄ClN₃S
• CAS Number: 531-53-3
• Molecular Weight: 291.80
• Colour Index Number: 52005
• Beilstein: 3922287
• EC Number: 208-510-7
• MDL number: MFCD00012112
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891073
• NACRES: NA.47

Properties

• form: powder or crystals
• Quality Level: 200
• composition: Dye content, ≥70%
• color: green to very dark green
• mp: 290 °C (dec.) (lit.)
• solubility: water: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• InChI key: NALREUIWICQLPS-UHFFFAOYSA-N

Description

Application

Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides.

Biochem/physiol Actions

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.

Caution

Handle and store under argon. Glove bag item.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves