M4530

Medium 199

With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): M199 Medium, TCM 199
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: yes; L-glutamine: yes; HEPES: no; Earle’s salts (5% CO₂): yes; phenol red: yes; sodium pyruvate: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Complete liquid version of the original M 199 formulation published by Morgan.

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

• as a suspension media to maintain the primary neuronal cultures
• as a component of the collagen gel for ex vivo embryonic heart culture
• to maintain the ovarian tissue sections as part of the slow-freezing protocol

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable