M3769
Medium 199
Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture
Synonym(s):
M199 Medium, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
Recommended Products
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
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General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
- to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
- to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)
Quantity
Formulated to contain 9.4 grams of powder per liter of medium.
Reconstitution
Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
also commonly purchased with this product
Product No.
Description
Pricing
supplement
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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