MABS2320
Anti-DNP Antibody, clone 1D10.4
Synonym(s):
DNP, Dinitrophenol
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1D10.4, monoclonal
mol wt
observed mol wt ~66 kDa
purified by
using protein G
species reactivity (predicted by homology)
all
packaging
antibody small pack of 100
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG2bκ
UniProt accession no.
storage temp.
2-8°C
Specificity
Clone 1D10.4 is a mouse monoclonal antibody that detects Dinitrophenol (DNP).
Immunogen
KLH-conjugated Dinitrophenol (DNP).
Application
Quality Control Testing
Evaluated by Western Blotting with DNP conjugated to BSA.
Western Blotting Analysis: A 1:500 dilution of this antibody detected DNP-conjugated BSA.
Tested Applications
ELISA Analysis: A representative lot of this antibody detected DNP in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with DNP conjugated to BSA.
Western Blotting Analysis: A 1:500 dilution of this antibody detected DNP-conjugated BSA.
Tested Applications
ELISA Analysis: A representative lot of this antibody detected DNP in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Dinitrophenols (DNPs) are highly toxic synthetic chemical compounds, classified as a mitochondrial uncouplers, as they disrupt the process of oxidative phosphorylation in mitochondria, leading to a significant increase in metabolic rate and heat production. DNPs can prevent adenosine triphosphate (ATP) synthesis, but permit electron transfer to proceed. Beside their commercial use as fungicides, herbicides, and insecticides, DNPs are useful tools to study cellular bioenergetics and the mechanisms of thermogenesis. It can be used as a reactant for catalytic reduction reactions, activation of carboxylic acids by converting them into dinitrophenyl (DNP) esters among others.
Physical form
Purified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.
Reconstitution
1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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