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Key Documents

SRP3158

Sigma-Aldrich

Sox2 TAT human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

36 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SOX2(6657)

General description

Sox2, also known as sex determining region Y (SRY)-box 2, is part of a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It contains 317 amino acids. The gene encoding this protein is localized on human chromosome 3q26.33. Recombinant human Sox2-TAT expressed in E. coli is a 36kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR).

Biochem/physiol Actions

Sex determining region Y (SRY)-box 2 (SRY-2) or Sox2 plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2kb upstream of the RAX promoter. The protein modulates stem cell function in embryonic and neural stem cells. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including fibroblast growth factor-4 (FGF-4), YES1 (a tyrosine-protein kinase) and ZFP206 (a transcription factor). Sox2 and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary as well as transformed cells.

Sequence

MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHMGGY GRKKRRQRRR

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Visite la Librería de documentos

Origins of specificity in protein-DNA recognition
Remo Rohs
Annual Review of Biochemistry, 79, 233-269 (2010)
Expression of the embryonic stem cell marker SOX2 in early-stage breast carcinoma
Claudia Lengerke
BMC Cancer, 11 (2011)
Benjamin Bonavida, Salem Chouaib
Resistance of Cancer Cells to CTL-Mediated Immunotherapy (2015)
SOX2 Is an Oncogene Activated by Recurrent 3q26.3 Amplifications in Human Lung Squamous Cell Carcinomas
Thomas Hussenet
PLoS ONE, 5(1), e8960-e8960 (2010)
Jun Guo et al.
Biochemical and biophysical research communications, 390(4), 1081-1086 (2009-11-11)
Recently, a series of exciting reports have revealed that terminally differentiated somatic cells can be reprogrammed to generate induced pluripotent stem (iPS) cells via overexpression of a cocktail of transcription factors such as Oct3, Sox2, Klf4, and c-Myc or Oct3

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