GE29-0485-86
HisTrap™ Excel
Cytiva 29-0485-86, pack of 1 mL
About This Item
Productos recomendados
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0485-86
availability
not available in North America
parameter
1 ml/min - 4 ml/min flow rate
5 bar
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
average diameter
90 μm
cleaning
2-14
1 of 4
Este artículo | MABC567 | HPA027532 | HPA014246 |
---|---|---|---|
unconjugated | - | unconjugated | unconjugated |
rabbit | mouse | rabbit | rabbit |
saturated ammonium sulfate (SAS) precipitated | purified immunoglobulin | affinity isolated antibody | affinity isolated antibody |
immunohistochemistry: 1:50-1:100, indirect ELISA: 1:1000 | immunohistochemistry: suitable (paraffin), western blot: suitable | immunohistochemistry: 1:200-1:500 | immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:1000-1:2500 |
General description
Features and Benefits
- Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- HisTrap excel columns allow direct purification of cell-free, unclarified samples
- HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
- HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.
Storage and Stability
Other Notes
Legal Information
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Warning
hcodes
Storage Class
3 - Flammable liquids
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Artículos
This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
Protocolos
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from GE Healthcare.
This page how to isolate proteins from inclusion bodies using GE Healthcare products.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.
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