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Key Documents

GE17-5168-01

Sigma-Aldrich

Mono S® 5/50 GL

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2SO3-

material

PEEK column

description

Ion Exchanger Type (Methyl sulfonate strong cation)

packaging

pkg of 1 mL

parameter

580 psi

bed size

5 mm × 50 mm

bed volume

1 mL

column I.D.

5.7 mm

matrix

monodisperse porous polystyrene/divinyl benzene beads
polymeric

matrix active group

methyl sulfonate phase

avg. part. size

10 μm

cleaning

1-14

working range

2-12

separation technique

cation exchange

General description

Mono S® is a strong cation exchanger prepacked with MonoBeads in a Tricorn column. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required. The column has 1 mL bed volume for small-scale purifications.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Features and Benefits

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono S is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Performing an isolation of recombinant protein complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Protocolos

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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