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GE17-0402-01

HiTrap® Protein A High Performance

Cytiva 17-0402-01, pack of 5 × 1 mL

Sinónimos:

Protein A Affinity Column

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

native protein A

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-0402-01

storage condition

(20% Ethanol)

parameter

flow rate
<4 mL/min flow rate
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10

working range

3-9

storage temp.

2-8°C

Categorías relacionadas

General description

HiTrap® Protein A HP is a prepacked Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

related product

Referencia del producto
Descripción
Precios

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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