GE28-9031-32

Protein A High Performance Spintrap^™

Cytiva 28-9031-32, pack of 16 columns

• UNSPSC Code: 41106500
• NACRES: NA.25

Propiedades

• ligand: native protein A
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• feature: wetted part: no
• packaging: pack of 16 columns
• manufacturer/tradename: Cytiva 28-9031-32
• storage condition: (20% Ethanol)
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
• working range: 3-9

Descripción

General description

protein A HP SpinTrap^™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose^™ HP.

Application

Protein A Sepharose^™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Features and Benefits

• For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
• Classic and cross-link protocols provide flexibility.
• Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
• Fast binding kinetics and high capacity provide high yield.
• Prepacked with Protein A Sepharose^™ High Performance for coupling of antibodies of IgG subclasses.
• Easy scale-up with HiTrap^® Protein A HP prepacked columns.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

Información de seguridad

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids