E7402

Elastin from human skin

insoluble powder

• Número de CAS: 9007-58-3
• EC Number: 232-701-4
• MDL number: MFCD03456696
• UNSPSC Code: 12352202
• NACRES: NA.61

Propiedades

• biological source: human skin
• Quality Level: 100
• form: powder
• technique(s): ligand binding assay: suitable
• UniProt accession no.: P15502
• storage temp.: 2-8°C
• InChI: 1S/C27H48N6O6/c1-9-17(6)23(32-24(36)19(13-15(2)3)30-18(7)34)26(38)29-14-21(35)31-22(16(4)5)27(39)33-12-10-11-20(33)25(37)28-8/h15-17,19-20,22-23H,9-14H2,1-8H3,(H,28,37)(H,29,38)(H,30,34)(H,31,35)(H,32,36)/t17-,19-,20-,22-,23-/m0/s1
• InChI key: DPUYCSDGMSDKKV-MKBYFEBXSA-N
• Gene Information: human ... ELN(2006)

Descripción

General description

Elastin is an extracellular matrix protein encoded by the gene ELN, which is mapped to human chromosome 7q11.23. Elastin is a major protein component of elastic fibres and is synthesized from the precursor protein called tropoelastin. Elastin is present in all the vertebrates except for the primitive fish. Elastin is majorly composed of glycine, proline and other hydrophobic residues.

Application

Elastin from human skin has been used as a substrate for the enzyme reaction with age-related NADH oxidase (arNOX) using fluorescein-conjugated tyramine.

Elastin is a structural protein which has been used in studies of chronic obstructive pulmonary disease (COPD). It may be used to investigate why there is a lack of repair for these proteins in patients with COPD.

Biochem/physiol Actions

Elastin is essential for resilience of arteries, and other elastic tissues like lungs and skin. Polymorphism in ELN gene causes intracranial aneurysm. Loss of function or haploinsufficiency of ELN gene leads to supravalvular aortic stenosis (SVAS). Frameshift mutations in ELN gene is implicated in congenital cutis laxa resulting in skin laxity and affect pulmonary and cardiovascular system.

Preparation Note

Prepared under mild extraction conditions to minimize peptide bond cleavage as described by Starcher, et al.

Analysis Note

Final purity determined by amino acid analysis and the percent insoluble residue remaining after digestion with porcine elastase.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)