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B2025

Sigma-Aldrich

Brilliant Blue G - Colloidal Concentrate

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

form

solution

Quality Level

200

storage temp.

room temp

General description

Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.

Application

Brilliant Blue G - Colloidal Concentrate has been used in the staining
  • protein fragments post in vitro pepsin digestion
  • ovarian tumor fluid proteome prior to profiling
  • the transcription factor (TFIID) complex from Saccharomyces cerevisiae

Reconstitution

The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

Related product

Referencia del producto
Descripción
Precios

G1041

EZBlue™ Gel Staining Reagent

pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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V Neuhoff et al.
Electrophoresis, 9(6), 255-262 (1988-06-01)
An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G-250 and R-250. The new method is based on addition of 20% v/v methanol and higher concentrations
Staining ability and biocompatibility of brilliant blue G: preclinical study of brilliant blue G as an adjunct for capsular staining
Hisatomi T, et al.
Archives of Ophthalmology, 124(4), 514-519 (2006)
Antonis Ververis et al.
Cellular and molecular life sciences : CMLS, 73(1), 163-184 (2015-07-15)
Katanins are microtubule (MT)-severing AAA proteins with high phylogenetic conservation throughout the eukaryotes. They have been functionally implicated in processes requiring MT remodeling, such as spindle assembly in mitosis and meiosis, assembly/disassembly of flagella and cilia and neuronal morphogenesis. Here
Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
Dyballa N and Metzger S
Journal of Visualized Experiments (2009)
A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins
Thomas K, et al.
Regulatory Toxicology and Pharmacology, 39(2), 87-98 (2004)
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Protocolos

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximum sensitivity.

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