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Merck

B8647

Sigma-Aldrich

Brilliant Blue R Concentrate

methanol solution

Sinónimos:

Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Fórmula empírica (notación de Hill):
C45H44N3NaO7S2
Número de CAS:
Peso molecular:
825.97
Colour Index Number:
42660
Beilstein/REAXYS Number:
5718025
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Brilliant Blue R Concentrate, suitable for SDS-PAGE, methanol solution

form

methanol solution

Quality Level

color

dark blue

suitability

suitable for SDS-PAGE

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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Application

Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).
For detection of protein bands following electrophoresis.

Reconstitution

After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.

Legal Information

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signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

57.2 °F - closed cup

flash_point_c

14 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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The effect of ethanol or hepatotoxin exposure on rat transferrin desialylation.
Lof K, et al.
Alcohol and Alcoholism (Oxford, Oxfordshire), 31, 445-451 (1996)
Crystallization of soluble proteins in vapor diffusion for x-ray crystallography.
Benvenuti M and Mangani S
Nature Protocols, 2, 1633-1651 (2007)
Overexpression of human apolipoprotein AI in transgenic rats and the hyperlipoproteinemia associated with experimental nephrosis.
Burkey BF, et al.
Journal of Lipid Research, 36.7, 1463-1473 (1995)
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The nucleation-promoting factor cortactin is expressed and promotes tumor progression and metastasis in various cancers. However, little is known about the biological role of cortactin in the progression of pancreatic ductal adenocarcinoma (PDAC). Cortactin and phosphorylated cortactin (Y421) were investigated
K Kubo
Analytical biochemistry, 213(2), 200-205 (1993-09-01)
In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Weber and Osborn, protein bands are often distorted by tailing at both ends, and the phenomenon is often called "edge tailing." This was eliminated by adding glycerol at

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