Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

125-50

SAFC

Collagen Coating Solution (50 ML)

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

form

liquid

shelf life

1 yr

shipped in

wet ice

storage temp.

2-8°C

General description

Collagen is a major fibrous, structural protein in animals. It is a key component of the extracellular matrix (ECM) and is predominantly present in the connective tissue, ligaments, tendons, and cornea. Collagens consist of three parallel left-handed polypeptide chains coiled to form a right-handed triple helix. They also have a globular domain.

Application

The coating concentration is 1 ml per 10 cm2 surface area of the cell culture ware: 2.5 ml for coating a T-25 flask or a 60 mm tissue culture dish. 7.5 ml for coating a T-75 flask or a 100 mm tissue culture dish.

Add Attachment Factor Solution to the flask and rock the flask gently to distribute the solution evenly to cover the whole culture surface. Coat the culture ware surface for 30 minutes at 37°C or 2 hours or overnight at room temperature. Remove Attachment Factor Solution by aspiration in a sterile hood. The coated flask can be used immediately or store at 4°C for up to 2 weeks.

Biochem/physiol Actions

Collagen is a basic unit for the growth and support of various cells and tissues in the body. Collagen may be involved in wound healing. Collagen might promote cell adhesion and proliferation when used as a coating on iron-manganese alloys (Fe-30%Mn) scaffolds in orthopedic applications.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Henry Bautista-Amorocho et al.
BMC molecular and cell biology, 22(1), 1-1 (2021-01-08)
Culturing primary epithelial cells has a major advantage over tumor-derived or immortalized cell lines as long as their functional phenotype and genetic makeup are mainly maintained. The swine model has shown to be helpful and reliable when used as a
Rajini Nagaraj et al.
Nanomaterials (Basel, Switzerland), 10(3) (2020-04-03)
Diabetic retinopathy (DR), Retinopathy of Pre-maturity (ROP), and Age-related Macular Degeneration (AMD) are multifactorial manifestations associated with abnormal growth of blood vessels in the retina. These three diseases account for 5% of the total blindness and vision impairment in the

Protocolos

Technical information for working with human follicle dermal papilla cells including thawing, subculturing and cryopreservation.

Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico