5162
Bovine Collagen Type I
from bovine flexor tendon, powder, suitable for cell culture, used for 3D gel formation
Sinónimos:
Collagen Protein, Collagen Solution, Collagen Type I
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
product name
Collagen, from bovine flexor tendon
biological source
bovine flexor tendon
sterility
non-sterile
assay
>96%
form
lyophilized powder (fibrous)
packaging
pkg of 1 g
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
binding specificity
Peptide Source: Elastin
Peptide Source: Fibronectin
Peptide Source: Laminin
shipped in
wet ice
storage temp.
room temp
Gene Information
bovine ... COL2A1(407142)
General description
This product is supplied as a lyophilized fibrous powder in a 1 gram package size. Bioburden and endotoxin levels are tested – this product is not considered sterile.
This collagen product is naturally cross-linked yielding a robust material for applications which require structure and strength. This product can be readily prepared into such forms as tissue scaffolds, foams, sponges, suspensions, coatings, putties, films and sheets but does not form hydrogels. Using typical cross-linking methods, this material can be tuned for optimal in vivo resorption. This collagen product is ideal for tissue engineering applications and uses with inorganic and biomaterials.
The product is provided in user-friendly packaging for use and storage. Avoid extended exposure to ambient environment since this material is hygroscopic.
This collagen product is naturally cross-linked yielding a robust material for applications which require structure and strength. This product can be readily prepared into such forms as tissue scaffolds, foams, sponges, suspensions, coatings, putties, films and sheets but does not form hydrogels. Using typical cross-linking methods, this material can be tuned for optimal in vivo resorption. This collagen product is ideal for tissue engineering applications and uses with inorganic and biomaterials.
The product is provided in user-friendly packaging for use and storage. Avoid extended exposure to ambient environment since this material is hygroscopic.
Preparation Note
Type I bovine collagen, insoluble lyophilized fibrous powder, is extracted from bovine flexor tendon with the raw material sourced from closed/controlled herds of animals. This product is very difficult to dissolve, and after following the below protocol you will end up with a viscous solution.
Note: The following procedure is based on solubilization of 1 gram of collagen in 100 ml to initially prepare a 10 mg/ml collagen suspension. Smaller quantities and volumes may be used but the same ratios of collagen and solutions should be used.
1. Weigh out 1 gram of collagen fibrous powder.
2. Reconstitute 1 gram of collagen with 50 ml of cold purified water (50% of the final volume).
3. Stir the collagen with the water continuously mixing for a minimum of 15 minutes until the collagen is fully wetted and the suspension appears to be a semi-solution.
4. Add 50 ml of cold 0.02 M HCl to the collagen mixture and stir for a minimum of 10 minutes. This will yield a collagen concentration of 10 mg/ml with the suspension continuing to appear as a semi-solution.
5. Measure the pH - the mixture should have a pH of 2 to 3.
6. Using Waring stick blender or equivalent, homogenize the collagen mixture for a minimum of 15 minute at a high speed ensuring that the collagen is fully homogenized. Ensure that the temperature of mixture does exceed 24°C. Upon completion, there should be no visual solids in the viscous suspension. Air bubbles with be prevalent.
7. To remove air bubbles, stir the solution on a stir plate and pull a vacuum on the suspension. This will remove the air bubbles.
8. At this point if a collagen concentration of less than 10 mg/ml is desired, the collagen can be diluted with 0.01 M HCl.
Note: The following procedure is based on solubilization of 1 gram of collagen in 100 ml to initially prepare a 10 mg/ml collagen suspension. Smaller quantities and volumes may be used but the same ratios of collagen and solutions should be used.
1. Weigh out 1 gram of collagen fibrous powder.
2. Reconstitute 1 gram of collagen with 50 ml of cold purified water (50% of the final volume).
3. Stir the collagen with the water continuously mixing for a minimum of 15 minutes until the collagen is fully wetted and the suspension appears to be a semi-solution.
4. Add 50 ml of cold 0.02 M HCl to the collagen mixture and stir for a minimum of 10 minutes. This will yield a collagen concentration of 10 mg/ml with the suspension continuing to appear as a semi-solution.
5. Measure the pH - the mixture should have a pH of 2 to 3.
6. Using Waring stick blender or equivalent, homogenize the collagen mixture for a minimum of 15 minute at a high speed ensuring that the collagen is fully homogenized. Ensure that the temperature of mixture does exceed 24°C. Upon completion, there should be no visual solids in the viscous suspension. Air bubbles with be prevalent.
7. To remove air bubbles, stir the solution on a stir plate and pull a vacuum on the suspension. This will remove the air bubbles.
8. At this point if a collagen concentration of less than 10 mg/ml is desired, the collagen can be diluted with 0.01 M HCl.
Other Notes
Simultaneously vacuum filter 12 samples for rapid analysis of retentate or filtrate. Numbered positions on the filter plate assure accurate sample tracking. Sealing stoppers maintain vacuum on unused sample cups. Accessory extension barrels increase sample volumes from 15 to 50 mL.
Applications
General Filtration of 15 to 50 mL Samples, Preparation for Scintillation Counting
XX2702550 1225 Sampling Manifold
Replacement Parts
YY2214257 Hand Knob
5162 Top Plate
XX2702509 Filter O-ring, silicone
5163 Support Plate
XX2702510 Support Screen, 25 mm, polypropylene
XX11000PP Ball Valve, glass-filled polypropylene
XX2504755 Tubing, 1/4 in. x 23 in., latex
Accessories
XX2702552 Chamber O-rings and Plug Set
XX2702555 Sample Cup Extension Barrel
XX6200006P Filter Forceps, blunt end, stainless steel
XX7100004 Tubing 3/16 in. I.D. x 140 cm, silicone
Applications
General Filtration of 15 to 50 mL Samples, Preparation for Scintillation Counting
XX2702550 1225 Sampling Manifold
Replacement Parts
YY2214257 Hand Knob
5162 Top Plate
XX2702509 Filter O-ring, silicone
5163 Support Plate
XX2702510 Support Screen, 25 mm, polypropylene
XX11000PP Ball Valve, glass-filled polypropylene
XX2504755 Tubing, 1/4 in. x 23 in., latex
Accessories
XX2702552 Chamber O-rings and Plug Set
XX2702555 Sample Cup Extension Barrel
XX6200006P Filter Forceps, blunt end, stainless steel
XX7100004 Tubing 3/16 in. I.D. x 140 cm, silicone
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Chiara Parravicini et al.
PloS one, 15(4), e0231483-e0231483 (2020-04-23)
The GPR17 receptor, expressed on oligodendroglial precursors (OPCs, the myelin producing cells), has emerged as an attractive target for a pro-myelinating strategy in multiple sclerosis (MS). However, the proof-of-concept that selective GPR17 ligands actually exert protective activity in vivo is
Sylvain Merel et al.
Food chemistry, 289, 259-268 (2019-04-09)
Ethoxyquin (EQ) is an additive present in fish feed and its fate in fish should be carefully characterized due to food safety concerns regarding this compound. Therefore, the objective of this work was to identify the transformation products (TPs) of
Yongjian Yue et al.
Journal of cellular biochemistry, 120(8), 12300-12310 (2019-02-28)
The disorders of hemostasis and coagulation were believed to be the main contributors to the pathogenesis of pulmonary thromboembolism (PTE), and platelets are the basic factors regulating hemostasis and coagulation and play important roles in the process of thrombosis. This
Amjad Alagha et al.
Journal of biomaterials science. Polymer edition, 31(13), 1689-1705 (2020-05-14)
This study aims to achieve the principles of tissue engineering using biopolymers to be applied in the field of vital endodontic treatment to stimulate stem cells and engineering and regeneration of dentin tissue. the polymer blend was loaded with the
Paola Bonsi et al.
EMBO molecular medicine, 11(1) (2018-12-16)
Dopamine D2 receptor signaling is central for striatal function and movement, while abnormal activity is associated with neurological disorders including the severe early-onset DYT1 dystonia. Nevertheless, the mechanisms that regulate D2 receptor signaling in health and disease remain poorly understood.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico