LRHSRMKB
Roche
KAPA LongRange
HotStart ReadyMix™, suitable for PCR, with dye
Sinónimos:
PCR
About This Item
Productos recomendados
Quality Level
usage
sufficient for 100 reactions
sufficient for 500 reactions
shelf life
≤18 mo.
feature
Long Range PCR
dNTPs included
hotstart
packaging
kit of 1.25 mL (100 x 25 μL rxn; KK3601)
kit of 6.25 mL (500 x 25 μL rxn; KK3602)
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
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General description
KAPA HotStart ReadyMix with dye Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template. The loading dye in the reaction buffer allows you to load PCR products directly onto the agarose gel with no extra steps.
Application
- Amplification of long targets and/or low amounts
- Standard short- and mid-range polymerase chain reaction (PCR)
- T-A cloning
- Targeted sample enrichment for next-generation DNA sequencing
- Complete genome amplification
Biochem/physiol Actions
Features and Benefits
- Amplification of targets with high sensitivity and specificity up to 15 kb
- High yields with low input DNA
Outperforms with complex targets:
- Exhibits high yield, greater sensitivity and specificity where competitor long-range systems fail
Quick Notes:
- KAPA LongRange HotStart is a blend of KAPA Taq HotStart and a proofreading DNA polymerase.
- Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA.
- Fidelity is 2–4 times better than Taq.
- For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
- Amplify mid- to long-range targets with high yield and sensitivity.
- 2X ReadyMix with dye contains two inert tracking dyes to allow loading of PCR products directly onto agarose gels for analysis.
Quality
Preparation Note
Other Notes
Legal Information
Solo componentes del kit
- KAPA Long Range DNA Polymerase or KAPA Long Range HotStart® DNA Polymerase 5 U/μL or 2.5 U/μL
- 1X KAPA Long Range Buffer
- dNTP Mix 0.3mM of each dNTP at 1X
- 2X KAPA LongRange HotStart® ReadyMix with dye
- MgCl2 at 1X 2 mM
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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