616407

TACE Substrate IV, Fluorogenic

• Sinónimos: TACE Substrate IV, Fluorogenic, TNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
• Fórmula empírica (notación de Hill): C₅₉H₉₃N₂₃O₂₀
• Peso molecular: 1444.51
• UNSPSC Code: 12352200

Propiedades

• assay: ≥95% (HPLC)
• Quality Level: 100
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: yellow
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex ~320 nm; λ_em ~420 nm
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

An internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (k_cat = 21.6 s^-1 when the substrate concentration is half the V_max, S_0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.

An internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (k_cat = 21.6 s^-1 and substrate concentration at half the V_max, S_0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity

Product does not compete with ATP.

Reversible: no

kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM

Warning

Toxicity: Standard Handling (A)

Sequence

Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Jin, G., et al. 2002. Anal. Biochem.302, 269.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable