220551

Chloramphenicol

Chloramphenicol, CAS 56-75-7, is a synthetic bacteriostatic antibiotic that inhibits the translation of RNA by blocking the peptidyltransferase reaction on ribosomes.

• Sinónimos: Chloramphenicol
• Fórmula empírica (notación de Hill): C₁₁H₁₂Cl₂N₂O₅
• Número de CAS: 56-75-7
• Peso molecular: 323.13
• MDL number: MFCD00078159
• UNSPSC Code: 12352200

Propiedades

• Quality Level: 100
• assay: ≥97% (by Assay)
• form: crystalline powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: gray to off-white
• solubility: water: 2.5 mg/mL; methanol: 35 mg/mL; ethanol: soluble
• shipped in: ambient
• storage temp.: 10-30°C
• InChI: 1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)
• InChI key: WIIZWVCIJKGZOK-UHFFFAOYSA-N

Descripción

General description

Synthetic bacteriostatic antibiotic that inhibits the translation of RNA by blocking the peptidyltransferase reaction on ribosomes.

Synthetic bacteriostatic antibiotic that inhibits the translation of RNA to protein by binding to the 50S ribosomal subunit, blocking the peptidyl transferase reaction. Chloramphenicol resistance is encoded by the resistance gene cat, whose product, chloramphenicol acetyltransferase, inactivates the antibiotic via acetylation. Typically used at 30-50 µg/ml for selection of chloramphenicol-resistant Escherichia coli.

Biochem/physiol Actions

Potency: ≥970 µg/mg

Primary Target
trasnslation of RNA

Other Notes

Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1, (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
Lu, J., and Jiang, C. 1993. Biochem. Biophys. Res. Commun.196, 12.
Saltarelli, M.J., et al. 1993. Virol.197, 35.
Holt, J.T. 1992. Ann. N.Y. Acad. Sci.660, 88.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, NY, p. 1.6, A.6.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• pictograms: GHS08,GHS05
• signalword: Danger
• hcodes: H318,H351,H361fd
• pcodes: P202 - P280 - P305 + P351 + P338 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Eye Dam. 1 - Repr. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3