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Merck
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Documentos clave

C3175

Sigma-Aldrich

Chloramphenicol-Water Soluble

powder, suitable for cell culture, BioReagent

Sinónimos:

Chloramphenicol, Chlornitromycin, Chlorocid, Chloromycetin, Cloramfenicol, D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, Chloromycetin, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]aceta, Kloramfenikol

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.76

Nombre del producto

Chloramphenicol-Water Soluble, powder, BioReagent, suitable for cell culture

product line

BioReagent

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 50-500 mg/mL (stock solution)
PBS: 50-500 mg/mL (stock solution)
other salt solutions: not recommended

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma

mode of action

protein synthesis | interferes

Application

Chloramphenicol-Water Soluble has been used:

  • as a supplement in Luria-Bertani (LB) medium to culture Escherichia coli
  • as a supplement in LB agar to culture Citrobacter rodentium
  • to culture Toxoplasma gondii

Biochem/physiol Actions

Chloramphenicol is a natural product found in Streptomyces venezuelae. It shows a broad range of antibiotic activity against Gram-negative and Gram-positive bacteria by binding to the peptidyl transferase loop of the ribosomal RNA to block the peptide bond formation.

Packaging

Package size based on chloramphenicol

Other Notes

Formulated to contain approx. 100 mg chloramphenicol per gram of powder; balance 2-hydroxypropyl-β-cyclodextrin.
Keep container tightly closed in a dry and well-ventilated place. Light sensitive. Keep in a dry place.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Repr. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

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Certificados de análisis (COA)

Lot/Batch Number
SLCK7972
SLCJ7035
SLCJ7032
SLCC7238
SLBW9823

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Chloramphenicol palmitate United States Pharmacopeia (USP) Reference Standard

USP

1107300

Chloramphenicol palmitate

A Chatzitakis et al.
Water research, 42(1-2), 386-394 (2007-08-19)
The photocatalytic degradation of Chloramphenicol, an antibiotic drug, has been investigated in aqueous heterogeneous solutions containing n-type oxide semiconductors as photocatalysts. The disappearance of the organic molecule follows approximately a pseudo-first-order kinetics according to the Langmuir-Hinshelwood model. It was observed
Taru Hilander et al.
Nucleic acids research, 46(2), 849-860 (2017-12-12)
Accuracy of protein synthesis is enabled by the selection of amino acids for tRNA charging by aminoacyl-tRNA synthetases (ARSs), and further enhanced by the proofreading functions of some of these enzymes for eliminating tRNAs mischarged with noncognate amino acids. Mouse

Artículos

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

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