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ISB1L

Sigma-Aldrich

InstantBlue™

Ultrafast Protein Stain

Synonym(s):

protein gel stain, protein stain

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

manufacturer/tradename

ISBT

technique(s)

protein staining: suitable

storage temp.

2-8°C

General description

InstantBlue™ is a ready-to-use solution stain that is specially formulated for ultra-fast (less than 15 min), sensitive (5 ng per band (BSA)) and safe detection of your proteins. Protein gels can be stained with InstantBlue in minutes without the need to wash, fix or destain. Gels can remain in the stain for weeks without concern. Only the proteins are stained resulting in well-defined blue bands on a highly transparent background. The reduction of background interference results in a better signal to noise ratio and may also have a positive impact on the overall resolution and sensitivity. The InstantBlue formulation is non-toxic and does not contain any methanol. Proteins stained using the InstantBlue stain are also compatible with mass spectrometry (MS) analysis.

Application

InstantBlue™ has been used in coomassie staining to validate the presence of proteins from SDS-PAGE gels. It has also been used for staining SDS-PAGE gels.

Components

contains Coomassie dye, ethanol, phosphoric acid and solubilizing agents in water. (Caution: Phosphoric acid is a corrosive liquid.)

Other Notes

Not available in Sweden and Denmark

Legal Information

InstantBlue is a trademark of Expedeon Protein Solutions

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
200312200
200513200
GR3363004-1
GR3363715-1
GR3363567-1

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This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot.

Sample Preparation & Gel Electrophoresis Troubleshooting

The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.

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The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

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