30915
Denhardt′s solution
BioReagent, for molecular biology, lyophilized powder
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About This Item
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grade
for molecular biology
product line
BioReagent
form
lyophilized powder
impurities
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
suitability
suitable for nucleic acid hybridization
storage temp.
2-8°C
General description
Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Application
Suitable to block non-specific binding sites in western, northern and Southern blot membranes
Components
Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).
Preparation Note
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.
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