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F9037

Sigma-Aldrich

Formamide

≥99.5% (GC), BioReagent, for molecular biology

Synonym(s):

Amide C1, Formic amide

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About This Item

Linear Formula:
HCONH2
CAS Number:
75-12-7
Molecular Weight:
45.04
Beilstein:
505995
EC Number:
200-842-0
MDL number:
MFCD00007941
UNSPSC Code:
12352111
PubChem Substance ID:
24894985

grade

for molecular biology

Quality Level

200

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

product line

BioReagent

Assay

≥99.5% (GC)

form

liquid

autoignition temp.

932 °F

technique(s)

electrophoresis: suitable
immunohistochemistry: suitable

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

suitability

suitable for nucleic acid hybridization

storage temp.

2-8°C

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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General description

Formamide is a clear liquid amide derived from formic acid. Formamide allows for the denaturation and renaturation of nucleic acids at room temperature.

Application

Formamide is suitable for use in hybridization buffers made using standard recipes. Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other applications include:

  • Preparation of brain sections of mice for BrdU immunohistochemistry
  • FISH analysis of HeLa cells.
  • Preparation of the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis(The Merck Index, 12th ed).
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Features and Benefits

  • Deionized for molecular biology applications
  • Packaged under argon gas in amber bottles to protect from oxidation
  • Used for DNA renaturation or DNA-RNA hybridization
  • Reduces thermal stability of double stranded nucleic acids

Suitability

  • nucleic acid hybridization
  • cryoprotectant and gel-stabilizer
  • destabilize nucleic acid duplexes
  • elimination of secondary structure of nucleic acids
  • preparation of hybridization buffers for RNA and DNA application

Other Notes

The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H351 - H360D - H373

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Laurent Geiser et al.
Journal of chromatography. A, 979(1-2), 389-398 (2002-12-25)
A nonaqueous capillary electrophoresis (NACE) method, coupled with either UV or electrospray mass spectrometry (ESI-MS), is described for the simultaneous analysis of seven beta-blockers. The same electrolyte, namely 25 mM ammonium formate and 1 M formic acid, was used with
R Temmerman et al.
Applied and environmental microbiology, 69(1), 220-226 (2003-01-07)
In order to obtain functional and safe probiotic products for human consumption, fast and reliable quality control of these products is crucial. Currently, analysis of most probiotics is still based on culture-dependent methods involving the use of specific isolation media
T Kaabache et al.
Analytical biochemistry, 232(2), 225-230 (1995-12-10)
The sensitivity of direct solution hybridization of hepatocytes solubilized in guanidium thiocyanate (GuSCN) for detecting alpha 1-acid glycoprotein and albumin mRNAs was studied. The sensitivity of detection was inversely correlated with the DNA concentration. Raising the hybridization temperature from 20
Jianwei Li
Journal of pharmaceutical sciences, 91(8), 1873-1879 (2002-07-13)
This article describes the proper selection of extraction solvents to eliminate interference from a polymer matrix to the quantitation of estradiol degradation products in a transdermal formulation by reversed-phase liquid chromatography. The separation is performed by gradient elution with acetonitrile
N C Pagratis
Nucleic acids research, 24(18), 3645-3646 (1996-09-15)
Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C
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Formamide Molecular Biology Reagent

Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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