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H9041

Sigma-Aldrich

Vascular Endothelial Growth Factor 121 human

VEGF121, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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About This Item

MDL number:
MFCD00286542
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

100

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤15 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 37 kDa (glycosylated)
trimer 50 kDa (glycosylated)

packaging

pkg of 10 μg

manufacturer/tradename

HumanZyme

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P15692

storage temp.

−20°C

Gene Information

human ... VEGFA(7422)

General description

HumanPro VEGF-121(vascular endothelial growth factor 121) is an isoform of VEGFA and is expressed in human HEK 293 cells as a disulfide linked, glycosylated cytokine with an apparent molecular mass of 37 kDa as a homodimer and 50 kDa as a homotrimer. VEGF-A exists in three different isoforms VEGF-121, VEGF165 is an angiogenic properties. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
There are five types of VEGF ligands identified in mammals, which exists as different splice variants.VEGFA is a dimeric glycoprotein consisting two monomers antiparallel to each other.

Biochem/physiol Actions

Vascular endothelial growth factor 121 (VEGF121) belongs to the PDGF/VEGF growth factor family. Many cell types secrete VEGF, and it is a potent angiogenic factor and mitogen that stimulates proliferation, migration, and formation of endothelial cells. VEGF stimulates permeabilization of blood vessels and is present in some tumors of the nervous system. VEGF is induced by hypoxia, oncogene mutations, and cytokines such as IL-1, IL-8, and TNF-α.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1X PBS

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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VEGF121b and VEGF165b are weakly angiogenic isoforms of VEGF-A
Catena R, et al.
Molecular Cancer, 9.1 (2010)
Vascular endothelial growth factor stimulates osteoblastic differentiation of cultured human periosteal-derived cells expressing vascular endothelial growth factor receptors.
Hah YS, Jun JS, Lee SG
Molecular Biology Reports (2010)
Transcriptome-wide Landscape of Pre-mRNA
Alternative Splicing Associated with Metastatic Colonization
Lu ZX, et al.
Molecular Cancer Research (2014)
Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability
Fukumura D, et al.
Proceedings of the National Academy of Sciences of the USA, 98.5, 2604-2609 (2001)
VEGF receptor signalling ? in control of vascular function
Olsson AK, et al.
Nature Reviews in Molecular and Cell Biology, 7.5 (2006)

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VEGF Pathway

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