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Key Documents

HL60 15-12

88120805

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About This Item

UNSPSC Code:
41106514

biological source

human blood

growth mode

Suspension

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian promyelocytic leukaemia

Cell Line Description

HL60 15-12 is a variant of HL60 which is capable of differentiating towards either neutrophils or monocytes with treatment of appropriate agents.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Subculture Routine

Maintain cultures between 2x100,000 to 1,000,000 cells/ml. NB: The cells will differentiate if the culture medium is allowed to become acidic. 5% CO2; 37°C. Freeze in 10% glycerol and 90% FBS.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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