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SRP5162

Sigma-Aldrich

4EBP1, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

BP-1, EIF4EBP1, MGC4316, PHAS-I

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About This Item

CAS Number:
185703-54-2
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~18 kDa

NCBI accession no.

NM_004095

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EIF4EBP1(1978)

General description

Eukaryotic translation initiation factor 4E binding protein 1 (E1F4EBP1) is part of the translation repressor protein family. The gene encoding it is localized on human chromosome 8p11-p12.

Biochem/physiol Actions

Eukaryotic translation initiation factor 4E binding protein 1 (E1F4EBP1) is involved in protein synthesis, cell growth and survival. In the unphosphorylated form, it suppresses the translation initiation of capped mRNAs, whereas in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eukaryotic translation initiation factor 4E (eIF4E) and this results in cap-dependent translation. The protein is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, E1F4EBP1 interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Certificates of Analysis (COA)

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Multiple interacting oncogenes on the 8p11-p12 amplicon in human breast cancer.
Yang ZQ
Cancer Research (2006)
4E-BP1, a multifactor regulated multifunctional protein.
Qin X
Cell Cycle (2016)
Stabilization of p21 by mTORC1/4E-BP1 predicts clinical outcome of head and neck cancers.
Llanos S
Nature Communications (2016)
K Tsukiyama-Kohara et al.
Genomics, 38(3), 353-363 (1996-12-15)
Two related eukaryotic initiation factor-4E binding proteins (4E-BP1 and 4E-BP2) were recently characterized for their capacity to bind specifically to eIF4E and inhibit its function. Here, we determined the cDNA sequence, tissue distribution, genomic structure, and chromosome localization of murine
A Pause et al.
Nature, 371(6500), 762-767 (1994-10-27)
The cloning is described of two related human complementary DNAs encoding polypeptides that interact specifically with the translation initiation factor eIF-4E, which binds to the messenger RNA 5'-cap structure. Interaction of these proteins with eIF-4E inhibits translation but treatment of
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