SRP5005
CDC25A, active, GST tagged human
recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
CDC25A2
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
recombinant
expressed in baculovirus infected Sf9 cells
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
26-36 nmol/min·mg
mol wt
~94 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CDC25A(993)
General description
CDC25A (also known as cell division cycle 25 homolog A) is a member of the CDC25 family of phosphatases that are required for progression from G1 to the S phase of the cell cycle. CDC25A can activate the cyclin-dependent kinase CDC2 (also known as CDK1) by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A overexpression is detected in human cancers and this may contribute to the tumorigenesis process. CDC25A is degraded by moderate heat shock and this degradation is protected by HSP90.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Sibylle Madlener et al.
Human molecular genetics, 18(11), 1990-2000 (2009-03-18)
The effects of heat shock (HS; 42 degrees C) on the cell cycle and underlying molecular mechanisms are astonishingly unexplored. Here, we show that HS caused rapid Cdc25A degradation and a reduction of cell cycle progression. Cdc25A degradation depended on
N Mailand et al.
Science (New York, N.Y.), 288(5470), 1425-1429 (2000-05-29)
To protect genome integrity and ensure survival, eukaryotic cells exposed to genotoxic stress cease proliferating to provide time for DNA repair. Human cells responded to ultraviolet light or ionizing radiation by rapid, ubiquitin- and proteasome-dependent protein degradation of Cdc25A, a
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