S9626
Sulfatase from Helix pomatia
Type H-1, sulfatase ≥10,000 units/g solid
Synonym(s):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
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About This Item
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type
Type H-1
Quality Level
form
powder
sulfatase activity
≥10,000 units/g solid
secondary activity
≥300 unit/mg solid β-glucuronidase (at pH 5.0)
mol wt
~85 kDa by gel filtration
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
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Application
Sulfatase from Helix pomatia has been used in:
- sample extraction
- sulfatase assays
- enzymatic hydrolysis
- extract preparation for glucosinolate analysis
Biochem/physiol Actions
Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).
substrate
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
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