G0751

β-Glucuronidase from Helix pomatia

Type H-1, partially purified powder, ≥300,000 units/g solid

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• Enzyme Commission number: 3.2.1.31 (BRENDA, IUBMB)
• EC Number: 232-606-8
• MDL number: MFCD00130629
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type H-1
• Quality Level: 200
• form: partially purified powder
• specific activity: ≥300,000 units/g solid
• secondary activity: ≥10,000 units/g solid sulfatase
• storage temp.: −20°C

Description

General description

β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H334
• Precautionary Statements: P261 - P280 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves