M1071

Anti-Mucolipin-3 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-MCOLN3, Anti-MLN3, Anti-TRPML3
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~75 kDa
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): western blot: 0.5-1 μg/mL using HEK-293T cells expressing human mucolipin-3
• UniProt accession no.: Q8TDD5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MCOLN3(55283) ; mouse ... Mcoln3(171166) ; rat ... Mcoln3(308022)

Description

General description

Mucolipin-3 (MLN3) belongs to mucolipin subfamily. It consists of six transmembrane domain (TMD), with a putative pore region between TMD5 and TMD6 and cytosolic N- and C-termini. MLN3 is a Ca²⁺ permeable cation channel, which is expressed in the plasma membrane, endosomes, lysosomes and autophagosomes.

Specificity

Additional bands may be observed at ~150 kDa and ~50 kDa due to aggregation and degradation, respectively.

Immunogen

synthetic peptide corresponding to amino acids 529-542 of human mucolipin-3. This sequence is identical in mouse and rat mucoplipin-3.

Application

Anti-Mucolipin-3 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Mucolipin-3 (MLN3) regulates endocytic pathway and lysosomal integrity. It modulates membrane trafficking and autophagy. Abnormalities of transient receptor potential cation channel, mucolipin subfamily, member 3 (TRPML3) are linked to early deafness, pigmentation abnormalities, and perinatal lethality in mice. Mutations in mouse mucolipin-3 encoded by the MCOLN3 gene, are associated with deafness and pigmentation defects in varitint-waddler mice.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves