CRISPRI10X

10X CRISPRi Feature Barcode Optimization Kit

Properties

• packaging: pkg of 8 vials (20µL for each subpool and 4x50µL aliquots of the effector, 5 kit components)
• application(s): CRISPR
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

This kit includes pooled virus representing each of the 2 possible capture sequences and 2 position combinations (capture sequences 1 or 2, in the stem-loop or 3 position). Present within each pool are 2 CRISPRi sgRNAs, 1 targeting RAB1A and 1 non-targeting control sgRNA. Each pool is provided ready to use at 1x10⁶ TU/mL, sufficient enough to cover 2x10⁶ cells at an MOI of 0.5. The purpose of the optimization kit is to set up a pilot screen to determine the best capture sequence and position configuration before a custom pool is ordered.

We have an exclusive partnership with 10x Genomics & our compatible CRISPRi pools are a perfect complementary product for target gene validation following a whole genome screen.

The 10X CRISPRi Feature Barcode Optimization kit is ideally suited for setting up the screen assay and if desired capture sequence optimization before using the Custom 10x Compatible CRISPRi library pools. For more information about single cell screening with CRISPR and 10x genomics Click Here.

Application

• Optimization kit for 10x CRISPRi screening
• Pooled CRISPR Screening with 10x Genomics Compatibility
• Strong transcriptional inhibition in multiple cell lines
• Creation of cell lines stably expressing KRAB-dCas9
• Validated positive and negative controls

Features and Benefits

• Focus on your research, and we will generate your lentivirus screening library
• No need for a proxy barcode or risk of recombination
• Resolve complex gene regulatory networks critical for biomarker or drug target discovery
• Analysis of single-cell perturbations from a mixed population reduces the noise in pooled screens and allows for whole transcriptome interrogation of rare cells
• Simplicity: 10x Feature Barcode technology compatible lentivirus CRISPR pool
• Flexibility: Submit your gRNA pool or have our team select gRNAs for you Focus on your research, and we will generate your lentivirus screening library

Components

4 Subpools with a minimum concentration of 1x10⁶ TU/ml (via FACS assay)
CRISPRI02: RAB1A And Nontargeting Control With 10x Capture Sequence 1 Stem-Loop Position
CRISPRI03: RAB1A And Nontargeting Control With 10x Capture Sequence 1 3 Prime Position
CRISPRI04: RAB1A And Nontargeting Control With 10x Capture Sequence 2 Stem-Loop Position
CRISPRI05: RAB1A And Nontargeting Control With 10x Capture Sequence 2 3 Prime Position

1 Effector Construct with a minimum concentration of 1x10⁵ TU/mL (via FACS or CFU assay)
CRISPRIE: KRAB-dCAS9 CRISPRi Construct Lentiviral Transduction Particles

Principle

CRISPR lentiviral products are powerful research tools for discovering novel genes and cellular pathways essential to understanding human health and disease. As a world leader in lentiviral libraries′ production, we design and build custom pools to any specifications. We have partnered with 10x Genomics to bring innovative pooled screening products to enable discovery at single-cell resolution with this capability.
CRISPR-based functional genomics screening provides a broad overview of the genetic contributions to a measurable phenotype. Bulk screening, however, only provides limited functional information beyond the selectable phenotype. At the other end of the spectrum, the single-cell analysis provides high content information centered around genome-wide transcriptome profiling for candidate genes. We offer optimized, customized lentiviral vectors compatible with Chromium Single Cell Gene Expression utilizing Feature Barcode technology.

Legal Information

CRISPR Use License Agreement

Lentiviral, WPRE and Evrogen Fluorophore Licenses

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable