MAIPN4550
Multiscreen® 96 well Plate, hydrophobic PVDF membrane
pore size 0.45 μm, non-sterile
About This Item
material
PVDF membrane (hydrophobic)
acrylic
flat bottom wells
Quality Level
description
Non-sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for Avidin-biotin Linkages, DNA-binding Proteins, Protein Binding & PAMPA. Comes in a pack of 50.
sterility
non-sterile
product line
MultiScreen®
feature
hydrophobic
lid
manufacturer/tradename
MultiScreen®
parameter
50-250 μL sample volume (per well)
technique(s)
parallel artificial membrane permeation assay (PAMPA): suitable
filtration area
0.3 cm2
plate size
96 wells
well maximum volume
300 μL
working volume
50-250 μL
matrix
Immobilon®-P
pore size
0.45 μm pore size
binding type
high binding surface
shipped in
ambient
Related Categories
Application
- as artificial membrane support and a receiver plate in parallel artificial membrane permeation assays (PAMPA) to determine the permeabilities of oximes
- in enzyme-linked immunospot (ELISpot) assay
- in keyhole limpet hemocyanin (KLH)-specific ELISPOT for the identification of antigen (Ag)-specific cells
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Articles
This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.
This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes
This article details the benefits of combining solubility and a non-cell based permeability assay (Parallel Artificial Membrane Permeation Assay; PAMPA) into a single workflow in which a portion of the filtrate from the MultiScreen® Solubility filter plate is added to the donor compartment of the MultiScreen filter plate for the PAMPA analysis.
Protocols
Assays that predict passive absorption of orally administered drugs have become increasingly important in the drug discovery process. As previously described by Faller and Kansy such assays provide rapid, low cost and automation friendly methods to measure a compound’s passive permeability.
Related Content
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
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