ABT1493
Anti-Cofilin-1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Cofilin-1, 18 kDa phosphoprotein, Cofilin, non-muscle isoform, p18
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology)
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... CFL1(1072)
General description
Cofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.
Specificity
This rabbit polyclonal antibody targets cofilin-1 N-terminal end sequence conserved among bovine, human, mouse, porcine, and rat species.
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-termnal end sequence of human cofilin-1.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cofilin-1 in HeLa and NIH/3T3 cells.
Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated cofilin-1 from 0.5 mg of MCF-7 cell lysate.
This poylclonal antibody is also available as Alexa Fluor™ 488 conjugate (Cat. No. ABT1493-AF488) for direct immunofluorescent staining.
Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated cofilin-1 from 0.5 mg of MCF-7 cell lysate.
This poylclonal antibody is also available as Alexa Fluor™ 488 conjugate (Cat. No. ABT1493-AF488) for direct immunofluorescent staining.
Research Category
Cell Structure
Cell Structure
This rabbit polyclonal Anti-Cofilin-1 Antibody, Cat. No. ABT1493 has been validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting for the detection of cofilin-1.
Quality
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cofilin-1 in 10 µg of MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cofilin-1 in 10 µg of MCF-7 cell lysate.
Target description
~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: AB3842
Physical form
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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