HPLC Analysis of Proteins on BIOshell A160 Peptide C18
Materials
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Ascentisยฎ Express Peptide ES-C18, 2.7 ฮผm HPLC Column
2.7 μm particle size, L ร I.D. 15 cm ร 2.1 mmํ์ค ๋ฌผ์ง
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Ribonuclease A from bovine pancreas
for molecular biology, ≥70 Kunitz units/mg protein, lyophilizedSuperoxide Dismutase from bovine erythrocytes
lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuretCarbonic Anhydrase from bovine erythrocytes
≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powderCONDITIONS
column
BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)
mobile phase
[A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile
gradient
held at 0% B for 0.5 min; 0 to 100% B in 7 min
flow rate
0.3 mL/min
pressure
4743 psi (327 bar)
column temp.
35 ยฐC
detector
UV, 220 nm
injection
1 μL
sample
0.2 - 0.6 g/L in mobile phase A
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