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설명
Adsorption capacity (> 80 g/L IgG (static); >70 g/L IgG (dynamic at 5 min. residence time))
Clean in Place (CIP) (0.1 M NaOH)
Phase filter (Protein A)
제품 라인
TOYOPEARL®
형태
slurry
포장
bottle of 10 mL
제조업체/상표
Tosoh 23425
파라미터
3 bar max. pressure
기술
LPLC: suitable
기질
hydroxylated methacrylic polymer particle platform
기질 활성군
rProtein A phase
입자 크기
45 μm
공극 크기
1000 Å
pH
2-13
용량
>70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time)
>80 g/L adsorption capacity (IgG)(static)
분리 기술
affinity
저장 온도
2-8°C
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일반 설명
TOYOPEARL AF-rProtein A HC-650F is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH.
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.
규격
Clean in place with 0.1 M NaOH.
물리적 형태
72% (v/v) slurry in 20% (v/v) ethanol
법적 정보
Toyopearl is a registered trademark of Tosoh Corporation
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point (°F)
104.0 °F - closed cup
Flash Point (°C)
40 °C - closed cup
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