추천 제품
product name
TSKgel® UltraSW Aggregate HPLC Column, phase diol, L × I.D. 30 cm × 7.8 mm, 3 μm particle size
material
stainless steel
Agency
suitable for USP L20
suitable for USP L59
제품 라인
TSKgel®
제조업체/상표
Tosoh UltraSW Aggregate, 22856
파라미터
≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1 mL/min flow rate
10-30 °C temperature
120 bar max. pressure
기술
HPLC: suitable
길이 × I.D.
30 cm × 7.8 mm
기질
silica particle platform
기질 활성군
diol phase
입자 크기
3 μm
공극 크기
300 Å
작동 pH
2.5-7.5
분리 기술
size exclusion (SEC)
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일반 설명
The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best for aggregation, dimer, and fragmentation, making it the best method for heterogeneity studies.
TSKgel UltraSW Aggregate columns have a slightly larger pore size and smaller (3 μm) particle size than the industry-leading TSKgel G3000SWxl (5 μm) and SuperSW3000 (4 μm) columns. These 30 cm x 7.8 mm ID silica-based, stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates.
The calibration curve of the TSKgel UltraSW Aggregrate column shows a separation range up to around 2 million Da, which provides better resolution of aggregates/multimers of a mAb than the TSKgel SuperSW mAb columns..
TSKgel UltraSW Aggregate columns have a slightly larger pore size and smaller (3 μm) particle size than the industry-leading TSKgel G3000SWxl (5 μm) and SuperSW3000 (4 μm) columns. These 30 cm x 7.8 mm ID silica-based, stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates.
The calibration curve of the TSKgel UltraSW Aggregrate column shows a separation range up to around 2 million Da, which provides better resolution of aggregates/multimers of a mAb than the TSKgel SuperSW mAb columns..
물리적 형태
Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.
법적 정보
TSKgel is a registered trademark of Tosoh Corporation
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문서
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
프로토콜
Calibration Curves for TSKgel® SW mAb Columns
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