추천 제품
product name
Discovery® BIO Wide Pore C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Agency
suitable for USP L1
제품 라인
Discovery®
특징
endcapped
제조업체/상표
Discovery®
포장
1 ea of
라벨링 범위
9.2% Carbon loading
파라미터
0-70 °C temperature
400 bar pressure (5801 psi)
기술
HPLC: suitable
LC/MS: suitable
길이 × I.D.
15 cm × 4.6 mm
표면적
100 m2/g
표면 범위
3.6 μmol/m2
기질
silica particle platform
fully porous particle
기질 활성군
C18 (octadecyl) phase
입자 크기
3 μm
공극 크기
300 Å
작동 pH
2-8
분리 기술
reversed phase
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
애플리케이션
- Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals.: This study provides a detailed kinetic performance comparison of different types of reversed-phase HPLC columns, including the Discovery® BIO Wide Pore C18 HPLC Column. The research highlights its efficiency and resolution in separating complex protein biopharmaceuticals, making it a valuable tool for analytical chemists in biopharmaceutical analysis (Jaag et al., 2022).
- RP-LC x RP-LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases.: This paper investigates the use of reverse-phase liquid chromatography (RP-LC) with both totally porous and partially porous stationary phases, including the BIO Wide Pore C18 HPLC Column, to analyze tryptic digests. The findings demonstrate its robustness and high resolution in proteomics applications, enabling precise peptide mapping and protein characterization (Mondello et al., 2010).
법적 정보
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
가아드 카트리지
항체
제품 번호
설명
가격
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
문서
Supelco Preparative HPLC products for Pharmaceutical Development and Production
관련 콘텐츠
DiscoveryBIO Wide Pore C18 products offered by Sigma-Aldrich.
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