추천 제품
구성
bed wt, 100 mg
Quality Level
포장
pk of 108
기술
solid phase extraction (SPE): suitable
용적
1 mL
기질 활성군
WAX phase
응용 분야
food and beverages
분리 기술
ion exchange
일반 설명
Sample Matrix Compatibility: Organic or aqueous Solutions
- Weak anion exchanger ideal for extracting strong basic compounds that remain charged at all pH levels
- Unlike conventional weak anion exchange SPE phases such as -NH2 (aminopropyl) that have a pKa of 9-10, a pH ≤ 7 is required to protonate or ionize the stationary phase to facilitate analyte retention. Elution is typically done by increasing the pH to 11 resulting in neutralization of the SPE phase.
- 2-(2-pyridyl)-ethyl silica gel has a pKa of ~6. Therefore, analyte elution is feasible at a pH ≥ 7. This characteristic is important for extracting analytes that are not stable (e.g. hydrolyzes) at high pHs typically required for elution when using traditional weak anion exchangers.
- Ideal for extracting acyl-coenzyme A esters from tissue.
- For more information, please see: Minkler, P.E., Kerner, J., Ingalls, S.T., Hoppel, C.L., Novel isolation procedure for short-, medium-, and long-chain acyl-coenzyme A esters from tissue, Analytical Biochemistry 376 (2008) 275–276
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
문서
SPE retention mechanism in this case is based on the electrostatic attraction of charged functional groups of the analyte(s) to oppositely charged functional groups on the sorbent.
프로토콜
Retention occurs through polar interaction between the sorbent and analytes. Typical sample matrices that can be employed in normal-phase SPE include hydrocarbon or fatty oils diluted in a solvent like hexane, isooctane, chlorinated solvent, THF, diethyl ether, or ethyl acetate.
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