ZRT1374

Anti-TIM-3 Antibody, clone RMT3-23 ZooMAb^® Rat Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): Anti-DRP1 Antibody, clone 1E8 ZooMAb^® Rabbit Monoclonal, CD366, HAVcr-2, Hepatitis A virus cellular receptor 2 homolog, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIMD-3
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rat
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: RMT3-23, recombinant monoclonal
• 설명: recombinant, expressed in HEK 293 cells
• 제품 라인: ZooMAb^® learn more
• 형태: lyophilized
• 분자량: calculated mol wt 30.93 kDa; observed mol wt ~26 kDa
• 정제법: using protein G
• 종 반응성: human, rat
• 종 반응성(상동성에 의해 예측): mouse
• 포장: antibody small pack of 25
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2aκ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_599011
• UniProt 수납 번호: Q8VIM0
• 환경친화적 대안 카테고리: Aligned
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 유전자 정보: mouse ... Havcr2(171285)

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

특이성

Clone RMT3-23 is a ZooMAb^® rat recombinant monoclonal antibody that specifically detects TIM-3.

면역원

Recombinant fragment corresponding to the first 191 amino acids from of Mouse TIM-3 conjugated to the Fc portion of mouse IgG2a.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Rat thymus tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TIM-3 in Rat thymus tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TIM-3 in Human thymus tissue lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TIM-3 in L5178Y cells transfected with TIM-3.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected TIM-3 in one million L5178Y cells transfected with TIM-3.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Hepatitis A virus cellular receptor 2 homolog (UniProt: Q8VIM0; also known as HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, TIMD-3, T-cell immunoglobulin mucin receptor 3, TIM-3, T-cell membrane protein 3, CD366) is encoded by the Havcr2 (also known as Tim3, Timd3) gene (Gene ID: 171285) in murine species. TIM-3 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 20-193), a transmembrane domain (aa 194-214), and a cytoplasmic domain (aa 215-281). Its expression is observed in T-helper type 1 cells, but not in naïve T cells. However, its expression is up-regulated as they differentiate into T helper type 1 cells. It is also expressed by differentiated type 1 CD8+ cytotoxic T-cells, peritoneal exudate macrophages, monocytes, and splenic dendritic cells. It serves as a receptor for galectin-9 (LGALS9), phosphatidylserine, HMGB1, and CEACAM1. Binding of LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Phosphorylation of TIM-3 on tyrosine 256 is reported to increase by binding to LGALS9. Phosphorylation on tyrosine 256 and 263 also couples it to TCR signaling pathways. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sabatos-Peyton, CA., et al. (2018). Oncoimmunology. 7(2); e1385690; Gorman, JV., et al. (2014). J. Immunol. 192(7); 3133-3142; Lee, J., et al. (2011). Mol. Cell Biol. 31(19); 3963-3974; Jayaraman, P., et al. (2010). J. Exp. Med. 207(11)l 2343-2354).

물리적 형태

Purified recombinant rat monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

재구성

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable