ZRB2184

Anti-GSDME/DFN5 Antibody, clone 1H7 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): Gasdermin-E, ICERE-1, Inversely correlated with estrogen receptor expression 1, Non-syndromic hearing impairment protein 5
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 1H7, recombinant monoclonal
• 설명: recombinant, expressed in HEK 293 cells
• 제품 라인: ZooMAb^® learn more
• 양식: lyophilized
• 정제법: using Protein A
• 종 반응성: human
• 포장: antibody small pack of 25 μL
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: affinity binding assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG
• 에피토프 서열: N-terminal
• 단백질 ID 수납 번호: NP_001120925
• UniProt 수납 번호: O60443
• 환경친화적 대안 카테고리: Aligned
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

특이성

Clone 1H7 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Gasdermin-E (GSDME). It targets an epitope within 20 amino acids from the N-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Gasdermin E (GSDME).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSDME/DFN5 in A549 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GSDME/DFN5 in SH-SY5Y cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GSDME/DFN5 in Human small intestine tissue sections.

Flow Cytometry Analysis: 1 µg from a representative lot detected GSDME/DFN5 in one million A549 cells.

Western Blotting Analysis: 0.1 µg/mL from a representative lot detected GSDME/DFN5 in lysates from HeLa cells, HEK293 cells, HEK293 cells transfected with DFNA5, and HeLa treated with TNF and cyclohexamide. (Data courtesy of Dr Kohsuke Tsuchiya from Kanazawa University, Japan).

Affinity Binding Assay: A representative lot of this antibody bound GSDME/DFN5 peptide with a KD of 5.3 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Gasdermin-E (UniProt: O60443; also known as Inversely correlated with estrogen receptor expression 1, ICERE-1, Non-syndromic hearing impairment protein 5, GSDME) is encoded by the GSDME (also known as DFNA5, ICERE1) gene (Gene ID: 1687) in human. Gasdermin-E (GSDME) is a precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis. The full-length protein is inactive and upon cleavage by caspase 3 or granzyme B at aspartic acid 270, the N-terminal moiety is released (aa 1-270) that binds to membrane, homooligomerizes, and forms pores of 10 to 15 nm inner diameter, triggering pyroptosis. The N-terminal GSDME is a multi-pass membrane protein whose interaction with the C-terminal domain is important for autoinhibition in the absence of an activation signal.  GSDME expression is observed in cochlea and placenta. Lower levels of expression are reported in heart, brain, lung, liver, kidney, pancreas, and skeletal muscle. GSDME is also known to mediate secondary necrosis downstream of the mitochondrial apoptotic pathway and caspase-3 activation as well as in response to viral agents. It can suppress tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells. It may also play a role in the p53/TP53-regulated cellular response to DNA damage. Three isoforms of GSDME have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Zhang, Z., et al. (2020). Nature. 579(7799); 415-420; Wang, Y., et al. (2017). Nature. 547(7661); 99-103; Masuda, Y., et al. (2006). J. Hum. Genet. 51(8); 652-664).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

재구성

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable