추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
4B4, monoclonal
recombinant monoclonal
제품 라인
ZooMAb® learn more
양식
lyophilized
분자량
calculated mol wt ~118.17 kDa
observed mol wt ~95 kDa
종 반응성
human
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
특이성
Clone 4B4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human NLRP3 protein. It targets an epitope within 15 amino acids from the N-terminal region.
면역원
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal domain of human NLRP3.
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in Human Spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NLRP3 in Human Spleen tissue lysate.Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound NLRP3 peptides with a KD of 1.9 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NLRP3 in human tonsil tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NLRP3 in THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human Spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NLRP3 in Human Spleen tissue lysate.Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound NLRP3 peptides with a KD of 1.9 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NLRP3 in human tonsil tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NLRP3 in THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-NLRP3, clone 4B4 ZooMAb®, Cat. No. ZRB1762, is a recombinant Rabbit monoclonal antibody that specifically targets NLRP3 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
표적 설명
NACHT, LRR and PYD domains-containing protein 3 (UniProt: Q96P20; also known as Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, CLR1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, NLRP3) is encoded by the NLRP3 (also known as C1orf7, CIAS1, NALP3, PYPAF1) gene (Gene ID: 114548) in human. NLRP3 serves as the sensor component of the NLRP3 inflammasome and plays a crucial role in innate immunity and inflammation. It is predominantly expressed in macrophages and its expression is also observed in dendritic cells, B- and T-cells, and in lung epithelial cells. NLRP3 can be induced by activators of Toll-like receptors, bacterial lipopolysaccharides, and by TNF. In response to pathogens and other damage-associated signals, it initiates the formation of the inflammasome polymeric complex that is composed of NLRP3, PYCARD and CASP1. Recruitment of proCASP1 to the inflammasome is shown to promote its activation and CASP1-catalyzed IL-1B and IL-18 maturation and secretion in the extracellular milieu. Under resting conditions, NLRP3 is autoinhibited and it is activated in response to several signals, including extracellular ATP, reactive oxygen species, K+ efflux, crystals of monosodium urate or cholesterol, and amyloid-beta fibers. Independently of inflammasome activation, it is reported to regulate the differentiation of T helper 2 (Th2) cells and is shown to play a role in Th2 cell-dependent asthma and tumor growth. NLRP3 undergoes polyubiquitination at lysine 48 and 63. However, this ubiquitination does not lead to its degradation, but inhibits inflammasome activation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
물리적 형태
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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