추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
2I22, recombinant monoclonal
monoclonal
제품 라인
ZooMAb® learn more
형태
lyophilized
분자량
calculated mol wt 343.91 kDa
observed mol wt ~350 kDa
종 반응성
mouse, human
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... LAMA2(3908)
mouse ... Lama2(16773)
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
특이성
Clone 2I22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Laminin subunit alpha-2. It targets an epitope within 18 amino acids from the N-terminal region.
면역원
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Laminin 2 alpha.
애플리케이션
Anti-Laminin a2, clone 2I22 ZooMAb, Cat. No. ZRB1023, is a recombinant Rabbit monoclonal antibody that specifically targets Laminin 2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blo
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Laminin 2 in human heart tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Laminin 2a peptide with a KD of 1.0 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin 2 in AC16 and C2C12 cells.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Laminin α2 in human heart tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Affinity Binding Assay: A representative lot of this antibody bound Laminin 2a peptide with a KD of 1.0 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin 2 in AC16 and C2C12 cells.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Laminin α2 in human heart tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
표적 설명
Laminin a2 (UniProt: P24043; also known as Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain) is encoded by the LAMA2 (also known as LAMM) gene (Gene ID: 3908) in human. Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains that are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Laminins mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Thus far five alpha, three beta, and three gamma subunits are known, which assemble into at least 15 different laminin molecules. Laminin a2 is a glycoprotein with multiple EGF-like domains and is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off. It is expressed in multiple tissues, including placenta, striated muscle, cardiac muscle, pancreas, lung, spleen, skin, bone, and some regions of the brain. Laminin a2 binds to cells via a high affinity receptor and mediates the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Mutations in LAMA2 gene have been linked Merosin-deficient congenital muscular dystrophy 1A (MDC1A) and limb-girdle muscular dystrophy that results in muscle weakness and atrophy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
물리적 형태
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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