Y1771

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

• 동의어(들): Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements
• MDL number: MFCD01095679
• UNSPSC 코드: 41106212
• NACRES: NA.85

속성

• Quality Level: 200
• 형태: powder
• 기술: transformation: suitable
• 응용 분야: microbiology
• 저장 온도: room temp

설명

애플리케이션

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

• the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
• Yarrowia lipolytica PO1f strain positive transformants
• CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

기타 정보

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

제조 메모

Dissolve 1.46 g of supplement per liter of medium.

안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H318
• 예방조치 성명서: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable