X0879

Anti-XPB antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-BTF2, Anti-ERCC3, Anti-Excision repair cross-complementing rodent repair deficiency, complementation group 3
• MDL number: MFCD02263308
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen 90 kDa
• 종 반응성: human, rat, mouse
• 농도: ~1 mg/mL
• 기술: indirect immunofluorescence: 10-20 μg/mL using HeLa cells fixed with paraformaldehyde-Triton; western blot: 1-2 μg/mL using HepG2 cell lysates
• UniProt 수납 번호: P19447
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ERCC3(2071) ; mouse ... Ercc3(13872) ; rat ... Ercc3(291703)

설명

일반 설명

XPB (Xeroderma pigmentosum complementation group B) is located on the human chromosome at 2q14.3. TFIIH (General transcription and DNA repair factor IIH helicase) consists of nine protein subunits, XPB (ERCC3) being one of them. It is an ATP-dependent DNA helicase of 89 kDa. The XPB is localized in the neurons.

면역원

synthetic peptide corresponding to amino acids 765-782 of human XPB, also known as ERCC3, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the mouse and rat sequences in one and three amino acids respectively.

애플리케이션

Anti-XPB antibody has been used in immunoblotting andimmunofluorescence.

생화학적/생리학적 작용

XPB (General transcription and DNA repair factor IIH helicase subunit XPB) is involved in DNA repair and transcription. Phosphorylation on Ser⁷⁵¹ of XPB inhibits TFIIH repair activity, leaving its transcription function intact. Mutations in the gene encoding XPB causes Xeroderma Pigmentosum, Cockayne′s syndrome, and trichothiodystrophy.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)