콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

TRUE3

Sigma-Aldrich

TrueGel3D Hydrogel Kits

FAST-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker

동의어(들):

Gel kits

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

배송 상태

dry ice

저장 온도

−70°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
TrueGel3D® provides a high degree of control in a 3D hydrogel environment, providing greater consistency. It enables to fine-tune the microenvironments around the tissues or cells under study.

애플리케이션

TRUE3-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been used to study the effect of mechanic stimulus over tumor development, tumor progression and cell differentiation.

특징 및 장점

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Contains MMP-cleavable crosslinker
  • Allow cell recovery and post-culture analyses
  • Flexibility in modulating gel stiffness
  • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)

성분

TRUE3-1KT contains lyophilized FAST-DEXTRAN (polymer) and CD cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

주의사항

Avoid prolonged exposure of CD cell-degradable crosslinker to air.

법적 정보

TRUEGEL3D is a registered trademark of Merck KGaA, Darmstadt, Germany

픽토그램

Corrosion

신호어

Warning

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

How some labs put more bio into biomaterials.
Vivien Marx
Nature methods, 16(5), 365-368 (2019-05-02)

문서

TrueGel3D™ synthetic hydrogel can be used to study co-culture of tumor and stromal cells.

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

프로토콜

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimic the native tissue environment.

관련 콘텐츠

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.