추천 제품
형태
buffered powder
품질
Microbiologically tested.
일반 설명
The tryptose component of Tryptose Phosphate Broth is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein.
애플리케이션
In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Tryptose Phosphate Broth has been used as a component:
- of Leibovitz L-15 medium for the culture of BME26 tick embryo cell line
- of M199 medium for the preparation of chick embryo fibroblasts (CEFs)
- of Glasgow′s minimum essential medium (GMEM) for culturing baby hamster kidney (BHK-21) before transfection
생화학적/생리학적 작용
Tryptose Phosphate Broth provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious micro-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.
성분
Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.
또한 이 제품과 함께 일반적으로 구입
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes
Chorev DS, et al.
Nature Protocols, 15(5), 1690-1706 (2020)
Dror S Chorev et al.
Nature protocols, 15(5), 1690-1706 (2020-04-03)
Recent applications of mass spectrometry (MS) to study membrane protein complexes are yielding valuable insights into the binding of lipids and their structural and functional roles. To date, most native MS experiments with membrane proteins are based on detergent solubilization.
S N Saha et al.
Vaccine, 7(4), 357-363 (1989-08-01)
Studies were undertaken to develop a cheaper medium with indigenous sources of peptone and casein hydrolysate for continuous culture of BHK-21 (suspension) cells and production of foot-and-mouth disease (FMD) vaccine. Eleven batches of experimental media were prepared using different indigenous
A I Josemans et al.
Annals of the New York Academy of Sciences, 969, 141-146 (2002-10-17)
The in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, was first achieved in 1985. Culture media were usually supplemented with serum and tryptose phosphate broth, both undefined components, contributing to great variability. Recently, we
S M Deutschmann et al.
Enzyme and microbial technology, 16(6), 506-512 (1994-06-01)
Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied
문서
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
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