T6451

Anti-Twist1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-Twist homolog 1 (acrocephalosyndactyly 3, Saethre-Chotzen syndrome), Anti-Twist related protein 1 (H-twist)
• MDL number: MFCD08705531
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~26 kDa
• 종 반응성: rat, human, mouse
• 포장: antibody small pack of 25 μL
• 농도: ~1 mg/mL
• 기술: indirect immunofluorescence: 1-2 μg/mL using human osteosarcoma MG-63 cells; western blot (chemiluminescent): 0.5-1 μg/mL using human 293T cells expressing recombinant human Twist1
• UniProt 수납 번호: Q15672
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TWIST1(7291) ; mouse ... Twist1(22160) ; rat ... Twist1(85489)

설명

일반 설명

Twist is encoded by the gene mapped to human chromosome 7p21.1. The encoded protein is a basic helix-loop-helix (bHLH) transcription factor. Twist 1 belongs to the Twist gene family and is mainly expressed in the suture mesenchyme.

특이성

Anti-Twist1 antibody recognizes human Twist1. Detection of the Twist1 band by immunoblotting is specifically inhibited with the immunizing peptide.

면역원

synthetic peptide corresponding to amino acids 12-27 of human Twist1 with a C-terminal added cysteine, conjugated to KLH. The corresponding peptide sequence is identical in both rat and mouse.

애플리케이션

Anti-Twist1 antibody is suitable for use in detecting Twist1 via in immunochemical applications such as immunoblotting (~26 kDa) and immunofluorescence.
Immunoblotting: a working antibody concentration of 0.5-1 mg/mL is recommended using extracts of human 293T cells expressing recombinant human Twist1 and a chemiluminescent detection reagent.

Anti-Twist1 antibody produced in rabbit has been used in:

• immunohistochemistry
• immunofluorescence
• immunoblotting
• chromatin immunoprecipitation (ChIP)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

생화학적/생리학적 작용

Twist is required for mesoderm formation. Twist 1 controls embryonic morphogenesis. It also plays a key role in metastasis by inducing an epithelial-mesenchymal transition (EMT). During an EMT, Twist stimulates the expression of mesenchymal markers, such as fibronectin and N-cadherin. In addition, it also blocks E-cadherin expression inhibiting cell-cell adhesion and therefore inducing infiltrative tumor growth in several human carcinomas. Twist1 is required in head mesenchyme for cranial neural tube morphogenesis in mice. Twist1 is involved in osteoblast differentiation and maturation. Increased expression of Twist 1 in breast cancer cells induce angiogenesis and leads to chromosomal instability. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome, an autosomal dominant defect characterized by minor skull and limb anomalies.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable